No orthologue was found in the opisthorchis viverrini genome archived. Opisthorchis viverrini and other foodborne trematode infections are major health problem in thailand, the lao peoples democratic republic, vietnam and cambodia. The distribution of the vitelline glands is also different. Immunological analysis of opisthorchis and clonorchis antigens volume 64 issue 2 s. Jan, 2012 read rapid detection and differentiation of clonorchis sinensis and opisthorchis viverrini eggs in human fecal samples using a duplex realtime fluorescence resonance energy transfer pcr and melting curve analysis, parasitology research on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Opisthorchis viverrini and clonorchis sinensis a opisthorchis viverrini thailand is the most endemic country for opisthorchiasis due to o.
Edoardo pozio, istituto superiore di sanita, department of infectious, parasitic and immunemediated diseases 1. These results indicate that opisthorchis is associated with moderate to severe hepatobiliary disease in a considerable proportion of infected individuals. May first report and molecular identification of opisthorchis viverrini infection in human communities from lower myanmar win pa pa. Streptococcus bovis, clonorchis sinensis, opisthorchis viverrini, schistosoma haematobium, aspergillus flavus, aspergillus parasiticus, cancer, oncogenesis 1.
Clonorchis sinensis and opisthorchis viverrini are both important fishborne pathogens, causing serious public health problem in asia. Animal models for opisthorchis viverrini infection animal models for opisthorchis viverrini infection boonmars, thidarut. People are infected after eating raw or undercooked fish. Introduction out of all of the infectious diseases worldwide, there are only a few microorganisms that have a welldefined mechanism associated with oncogenesis.
The liver and intestinal fishborne zoonotic trematodes flukes are important parasites of humans and animals and are estimated to infect more than 18 million people, especially in asia. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a pdf plugin installed and enabled in your browser. Skip directly to site content skip directly to page options skip directly to az link centers for disease control and prevention. Molecular identification of clonorchis sinensis and. Liver fluke infection with opisthorchis viverrini, opisthorchis felineus and clonorchis sinensis remains a major public health problem in many parts of asia and eastern europe. Clonorchiasis whose symptoms are indistinguishable from opisthorchiasis is one of the most neglected tropical diseases which blight the lives of millions of people worldwide and threaten the health of several others 5, 6. Uncooked fish consumption among those at risk of opisthorchis. Detection of clonorchis sinensis circulating antigen in sera.
The antigenic profiles of the crude extract of clonorchis contained major proteins of 8, 2628, 3437, 43, and 70 kda, and those of opisthorchis 3437, 43, 70, and 100 kda. Chronic infection may lead to cholangiocarcinoma, a malignant cancer of the bile ducts. Since that time, new data have become available, these have been incorporated in the monograph, and taken into consideration in the present evaluation. Trematode parasites of the genera clonorchis, opisthorchis and metorchis, commonly referred to as liver flukes, are transmitted to humans and other mammals by the ingestion of fish infected with their larval stages which ultimately come from snails infected due to excreta. The present study developed an assay integrating realtime pcr and high resolution melting hrm analysis for the specific detection and rapid identification of c. The chinese liver fluke clonorchis opisthorchis sinensis is widely distributed in the far east. Files are available under licenses specified on their description page.
The flexibility and specificity make mlpa a potential tool for specific identification of infections by opisthorchid liver flukes in endemic areas. Humans contract the disease through consumption of raw or inadequately cooked freshwater fish containing the infective. Current status and perspectives of clonorchis sinensis and. However, tribendimidine has a similar egg reduction rate to praziquantel and leads to fewer adverse events and thus might complement praziquantel in o viverrini control programmes, particularly in settings coendemic for hookworm.
Epidemiology and prevalence of clonorchis sinensis and opisthorchis viverrini infection in humans. The trematode clonorchis sinensis chinese or oriental liver fluke is an important foodborne pathogen and cause of liver disease in asia. Winner of the standing ovation award for best powerpoint templates from presentations magazine. The ecology of the bithynia first intermediate hosts of. Opisthorchis sinensis definition of opisthorchis sinensis. In this study, we refer to the liver fluke from ducks as.
Opisthorchis viverrini ov, a human liver fluke, is a pathologically and economically important foodborne trematode. Alternatively, you can download the file locally and open with any standalone pdf reader. Worlds best powerpoint templates crystalgraphics offers more powerpoint templates than anyone else in the world, with over 4 million to choose from. The asiatic liver fluke, a species of trematodes family opisthorchiidae that in the far east infects the bile passages of humans and other fisheating animals. The tumorigenic liver fluke opisthorchis viverrini. Clonorchis sinensis informacion practica profesionales. Opisthorchis viverrini, opisthorchis felineus and clonorchis sinensis 1. Opisthorchis species are liver fluke parasites that humans can get by eating raw or undercooked fish, crabs, or crayfish from areas in asia and europe where the parasite is found, including thailand, laos, cambodia, vietnam, germany, italy, belarus, russia, kazakhstan, and ukraine. Clonorchis sinensis, opisthorchis spp, and metorchis spp. Opisthorchis definition of opisthorchis by medical. The fulllength cs1 cdna sequence was obtained by 5. The antigenic characterizations and serological reactions of human liver flukes, clonorchis sinensis and opisthorchis viverrini, were analyzed by immunoblot. Human clonorchiasis is a prevailing foodborne disease caused by clonorchis sinensis infection.
Application of a loopmediated isothermal amplification lamp. Clonorchis sinensis also known as opisthorchis sinensis and opisthorchis viverrini affect about 20 million people in china and south east asia. Clonorchis sinensis and clonorchiasis pdf free download. Adults then parasitize the peripheral intrahepatic bile ducts. This parasite lives in the liver of humans, and is found mainly in the common bile duct and gall bladder, feeding on bile. Current status of clonorchis sinensis and clonorchiasis in. Clonorchis sinensis, an oriental liver fluke, as a human biological. The morphology of the egg is very similar to that of other species of human liver flukes opisthorchis felineus and clonorchis sinensis as well as that of small intestinal flukes in the family. The two testes are located posterior to the ovary, and are highly brancheda feature which separates it from the related opisthorchis spp. Liver fluke infection caused by opisthorchis viverrini is a major public health problem in thailand and adjacent countries. Liver flukes infect the liver, gallbladder, and bile duct in humans.
Population structure of opisthorchis felineus trematoda and. Although man is the main host, the parasite also infects cats, dogs and other fisheating carnivores which probably serve as reservoirs of human infection. The draft genome of the carcinogenic human liver fluke. This page was last edited on 25 december 2019, at 21. The complete mitochondrial genomes of the liver flukes opisthorchis felineus and clonorchis sinensis trematoda molecular and biochemical characterizations of three fructose1,6bisphosphate aldolases from clonorchis sinensis. Opisthorchis definition of opisthorchis by medical dictionary. Opisthorchis viverrini and clonorchis sinensis are liver flukes a type of flatworm that are associated with an increased risk of cholangiocarcinomas. Animal models for opisthorchis viverrini infection. Opisthorchiasis is generally caused by opisthorchis felineus or o. Cancer and infectious causes scientific research publishing. Opisthorchis viverrini and clonorchis sinensis were considered by a previous iarc working group in 1994 iarc, 1994. These animals, which are believed to be the third most prevalent worm parasite in the world, are endemic to japan, china, taiwan, and southeast asia. Clonorchis sinensis wikipedia, a enciclopedia livre.
Tribendimidine has a slightly lower cure rate than praziquantel and noninferiority was not shown. In thailand and neighbouring countries, human opisthorchiasis is caused by o. Rapid detection of opisthorchis viverrini coprodna using. Frontiers molecular identification and phylogenetic. Cs1, a clonorchis sinensisderived serodiagnostic antigen. Cdc clonorchis biology centers for disease control. Clonorchis sinensis, the chinese liver fluke, is a human liver fluke in the class trematoda, phylum platyhelminthes. Identification and characterization of clonorchis sinensis cathepsin. Opisthorchiasis is a parasitic disease caused by species in the genus opisthorchis.
Pronunciation of clonorchis sinensis with 1 audio pronunciation, 1 meaning and more for clonorchis sinensis. This fluke is quite common in china, japan, korea, taiwan and vietnam among others. Clonorchis sinensis, the chinese liver fluke, is a liver fluke belonging to the class trematoda, phylum platyhelminthes. Clonorchis sinensis, opisthorchis viverrini and opisthorchis felineus are three closely related species that produce similar clinical manifestations in humans.
In 198081, the prevalence in the north, northeastern, centre. Liver fluke infection is recognized as the major risk. Population structure of opisthorchis felineus trematoda. All structured data from the file and property namespaces is available under the creative commons cc0 license. In addition to infectionassociated morbidity, infection with o. Pdf clonorchis sinensis and opisthorchis viverrini. In humans, it infects the common bile duct and gall bladder, feeding on bile. Clonorchis sinensis adults are flattened, lance shaped, and measure approximately 10 to 25 mm long by 3 to 5 mm wide. The adult flukes deposit fully developed eggs that are passed in the feces. We report a single, onestep pcr approach for detection and discrimination of clonorchis sinensis and opisthorchis viverrini in different lifestage forms adults, metacercariae, and eggs from fish intermediate hosts and from infected patients. Trematodes 2 liver and intestinal flukes trematodes2.
Opisthorchis viverrini an overview sciencedirect topics. Opisthorchis viverrini and clonorchis sinensis biological. In addition, genomic dna of clonorchis sinensis in fecal samples of infected rats was positively amplified by mlpa. The loopmediated isothermal amplification lamp assay has been applied for the detection of clonorchis sinensis dna. Efficacy and safety of tribendimidine versus praziquantel. These liver flukes are known to cause serious human diseases affecting bile ducts and the gall bladder. Theyll give your presentations a professional, memorable appearance the kind of sophisticated look that. Trematodes flukes opisthorchis viverrini southeast asian liver fluke and opisthorchis felineus cat liver fluke. Infections with the opisthorchid liver flukes clonorchis sinensis, opisthorchis viverrini, and o. The oral and ventral suckers acetabulum are relatively small. If the inline pdf is not rendering correctly, you can download the pdf file here. Low divergence of clonorchis sinensis in china based on. Human infection follows consumption of metacercariae in raw or undercooked freshwater fish.
Infection with the parasite is called opisthorchiasis. Infections with the opisthorchid liver flukes clonorchis sinensis, opisthorchis. Clonorchis sinensis and opisthorchis species are important liver flukes of oriental or chinese origin 14. May first report and molecular identification of opisthorchis viverrini infection in human communities from lower myanmar win pa pa aung 0 1 2 thi thi htoon 0 2 htay htay tin 0 2 kyi kyi thinn 0 2 oranuch sanpool 0 1 2 jurairat jongthawin 0 1 2 lakkhana sadaow 0 1 2 issarapong phosuk 0 1 2 rutchanee rodpai 0 1 2 pewpan m. After ingestion by a suitable snail first intermediate host, the eggs release miracidia, which undergo in the snail several developmental stages sporocysts, rediae. This species uses common cyprinoid fishes as second intermediate hosts as does opisthorchis viverrini, with which it is sympatric in this province. Dec, 2018 opisthorchiasis is an infection caused by either of the two parasite worms, the cat liver fluke, or scientifically called opisthorchis felineus also known as opisthorchis tenuicollis, or the southeast asian liver fluke called as opisthorchis viverrini. A highly significant positive relationship between the intensity of liver fluke worm burden and the severity of biliary tract disease within individuals is reported. Siberia and south east asia these diseases are of both economic and public health concern.
Opisthorchis viverrini and clonorchis sinensis were considered by a previous iarc working group in 1994. Trematode parasites of the genera clonorchis, opisthorchis and metorchis, commonly referred to as liver flukes, are transmitted to humans and other mammals by the ingestion of fish infected with their larval stages which. Opisthorchis felineus rivolta, 1884 is a member of the triad of epidemiologically important fishborne liver trematodes, which also includes o. It was discovered by a british physician james mcconnell at the medical college hospital in calcutta kolkata in 1874. Clonorchis sinensis infection has been reported from many parts of east asia russia, japan, korea and china, whereas o.
Opisthorchis viverrini, common name southeast asian liver fluke, is a foodborne trematode parasite from the family opisthorchiidae that infects the bile duct. Early identification of the infection is essential to provide timely and appropriate chemotherapy to patients. Pdf opisthorchis viverrini and opisthorchis felineus. Race and consists of 733 nucleotides, with a coding sequence from 23619 that encodes a putative protein of 198 amino acids. Immunological analysis of opisthorchis and clonorchis.
Six primers targeting eight locations on the cytochrome c oxidase subunit 1 gene of c. Education and information about opisthorchis, biology and life cycle. In 1874, a 20 year old chinese carpenter was admitted to the medical college hospital. Clonorchis sinensis introduction to medical parasitology. Clonorchis sinensis, an oriental liver fluke, as a human biological agent carcinogen of cholangiocarcinoma. Nov 21, 2017 an opisthorchiid liver fluke was recently reported from ducks anas platyrhynchos in binh dinh province of central vietnam, and referred to as opisthorchis viverrinilike. Clonorchis sinensis is a fishborne trematode that inhabits the bile duct of mammals including humans. Clonorchis sinensis an overview sciencedirect topics. A related species, opisthorchis felineus, occurs in eastern europe and russia. These are liver flukes of cats, dogs, and other fisheating mammals, which serve as reservoir hosts. Development of a mitochondrialbased multiplex pcr for their identification and discrimination. Pdf clonorchis sinensis, an oriental liver fluke, as a human.
Pdf clonorchis sinensis and opisthorchis viverrini are clinically important small liver flukes because of their known association with development of. Updated molecular phylogenetic data for opisthorchis spp. Using nonoverlapping sliding windows along the genome, we found a random distribution of sequencing depth over areas with different gc content within a range of 30 to 60% covering more than 99. Infection by opisthorchis viverrini and other liver flukes in asia affect the poor and poorest people. Read rapid detection and differentiation of clonorchis sinensis and opisthorchis viverrini eggs in human fecal samples using a duplex realtime fluorescence resonance energy transfer pcr and melting curve analysis, parasitology research on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your. First report and molecular identification of opisthorchis. The three epidemiologically important opisthorchiidae liver flukes opisthorchis felineus, o. The tumorigenic liver fluke opisthorchis viverrini multiple. Clonorchis sinensis, opisthorchis viverrini, and o. Cholangiocarcinoma, clonorchiasis, clonorchis sinensis.
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